Banca de DEFESA: FRANCYELLY MONICKE BEZERRA DE MOURA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : FRANCYELLY MONICKE BEZERRA DE MOURA
DATE: 01/02/2024
TIME: 08:00
LOCAL: CECA - Campus de Engenharias e Ciências Agrárias
TITLE:

Influence of leukofiltration in controlling Escherichia coli in equine blood bags

KEY WORDS:

Blood bank, leukoreduction, bacteria, horse.

PAGES: 51
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Leukoreduction by filtration (LRF) is based on barrier retention and adsorption of white cells in the membrane, made of polymeric fiber in the form of a mesh with different pore sizes, thus acting as a filter. Transfusion-transmitted infections can occur from bacterial contamination of the donor's skin at the time of collection, unknown bacteremia in the donor, environmental contamination, or veterinary manipulation. The LRF technique can also be used to reduce the transmission of infectious agents, such as viruses, bacteria, or blood parasites. To evaluate the efficiency of leukoreduction by filtration in the quantitative control of Escherich-ia coli (E. coli) inoculated into equine blood bags during storage, 6 hematologically stable horses were used, from which blood was collected for study in 14 blood bags. type CPDA-1 and divided into two groups, seven bags each. The experimental group (LRF) was leukofil-tered after 2 hours of storage and the control group (no LRF) did not go through the filtration process. The samples were analyzed for Hemogram, Osmotic Fragility, Fibrinogen, Plasma Potassium, Plasma Glucose, Total Plasma Protein, Plasma Albumin, Percentage of Hemolysis, and Analysis of Bacterial Growth, being collected for laboratory analysis at the times: T0: before hemofiltration; T1: immediately after hemofiltration; T7: seven days after collection; T14: fourteen days after collection. The evaluation of the results allowed us to conclude that The human leukocyte filter was efficient in obtaining leukoreduced whole blood inoculated with Escherichia coli for the equine species, without quantitative reduction in the red series or increase in cell fragility. However, the filtered bags showed greater hemolysis during storage, observed by the increase in potassium and osmotic fragility. There was a significant reduction in the amount of E.coli, through the use of leukocyte filters and the observation of a reduction in the number of E.coli during storage, in both groups, suggesting the presence of other factors in bacterial control, in addition to leukocytes.

COMMITTEE MEMBERS:
Presidente - 1698460 - MARCIA KIKUYO NOTOMI
Interno(a) - 1640263 - KARLA PATRICA CHAVES DA SILVA
Externo(a) à Instituição - FABÍOLA PAES LEME - UFMG