Banca de QUALIFICAÇÃO: FRANCYELLY MONICKE BEZERRA DE MOURA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : FRANCYELLY MONICKE BEZERRA DE MOURA
DATE: 19/12/2023
TIME: 09:00
LOCAL: Unidade Educacional Viçosa
TITLE:

The reduction of Escherichia coli contamination through leukoreduction in stored equine blood bags.

KEY WORDS:

Blood bank, leukoreduction, bacteria, horse.

PAGES: 30
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Leukoreduction by filtration (LRF) is based on the retention by barrier and adsorption of the white cells in the membrane, made of polymeric fiber in the form of a mesh with different pore sizes, thus performing the function of a filter. This technique has also been used to reduce the transmission of infectious agents, such as viruses, bacteria, or blood parasites. This study aims to evaluate the efficiency of leukoreduction in the quantitative control of Escherichia coli inoculated in equine blood bags during storage. Blood was obtained from 6 healthy horses, and the amount collected was approximately 900 ml from each animal. The whole blood bags were refrigerated for 2 hours between 2° and 6 °C, each bag was inoculated with 1 ml of E. coli diluted in Brain Heart Infusion (BHI) broth, and then they were separated into two groups, experimental (n=7) and control (n=7). In the bags of the experimental group, leukocyte filters were used, while the bags of the control group remained unfiltered. Samples of 5 mL of blood were taken from each bag, 4.0 mL of which were placed in a tube without anticoagulant for immediate analysis, and the remaining 1 mL of the syringe was sent for microbiology. The tests took place at 4 different times: at the time of filtration on the day of collection (T0), after filtration (T1), on the 7th day (T2), and the 14th day (T3). Blood count, osmotic fragility, percentage of hemolysis, and bacterial culture were analyzed. Mean and standard deviation were determined and the results were compared using Tukey's test, considering a significant p-value <0.05. At the end of the process, the LRF blood had a 100% reduction in the number of leukocytes, since no white blood cells were identified in the automatic cell count, and the Neubauer chamber, a 98% reduction in bacteria during the 14 days, with 40% retained in the filtration screen and a reduction in the first seven days. The blood that didn't go through LRF had a bacterial reduction of 78% in the same period.

COMMITTEE MEMBERS:
Interno(a) - 1640263 - KARLA PATRICA CHAVES DA SILVA
Presidente - 1698460 - MARCIA KIKUYO NOTOMI
Interno(a) - 1670912 - PIERRE BARNABE ESCODRO