Banca de DEFESA: VANNESSA RODRIGUES TELES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : VANNESSA RODRIGUES TELES
DATE: 31/08/2023
TIME: 09:00
LOCAL: googlemeet
TITLE:

Antimicrobial and antioxidant profile of metabolites produced by filamentous fungi of the Caatinga biome

KEY WORDS:

Bioactive compounds, antimicrobial resistance, secondary metabolites,
fungal secondary metabolites.

PAGES: 35
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

The advent of antibiotics has revolutionized medical treatments, enabling the
development of invasive and surgical techniques with feasibility of recovery and
maintenance of the lives of individuals after the procedures, as well as the cure of diseases
before fatal. Nevertheless, excessive and irregular use, in addition to genetic mechanisms
developed by the microorganisms themselves, brought about microbial resistance,
growing problem that has become a global concern in the 21st century, bringing to the fore the
need for research for new antimicrobials. The search for new bioactive compounds
antimicrobial and antioxidant activities from natural sources have great
potential for application in human health. From this perspective, the Caatinga, because it is a biome
exclusively Brazilian, with unique climatic characteristics, can host fungi
producers of differentiated secondary metabolites. Thus, the objective was to evaluate the
antimicrobial and antioxidant potential of secondary metabolites produced by fungi
isolated filamentous liqueurs from the Caatinga of Alagoas. To this do so, 35
fungi, produced in solid culture medium Czapek Agar supplemented with
glucose 2% by incubation at 30 oC for 15 days. The extraction of secondary metabolites was
performed in ethyl ACETATE PA, followed by concentration in rotary stirrer and drying in
desiccator coupled to the vacuum pump, followed by production yield evaluation. The
extracts were resuspended in DMSO (100 mg/mL) and initially evaluated the
antibacterial and antifungal activities by disc diffusion and antioxidant assay by
FRAP and DDPH. The next steps will be the assessment of Minimum Injunction Concentrations
(MIC) of extracts with antimicrobial activity, in addition to the analysis of the effect of metabolites
antimicrobials in biofilms of Staphylococcus aureus and Candida albicans, by means of
cell viability test at different concentrations of extracts, as well as by
scanning optical microscopy. As partial results, the yield of the extracts
ranged from 45.00 mg to 173.00 mg, and the extracts showed different
colors such as 15 yellows, seven oranges and two roses. The fungal extract 3UVLFC1
antimicrobial activity against Staphylococcus aureus, while the samples
Penicillium sp. (2LCL1), Fusarium sp. (4LCEM2) and 4UVLFC2 demonstrated
against Candida abicans. Regarding antioxidant activity, the following samples showed
activity identified by frap and DPPH methods: 2LCL1, 4UVLFC2, 3UVLFC7,
4LCEM2, 7UVLFC2, 4UVLFC7, PLC6, Fusarium sp. (ALC3), 1UVLFC7 while the samples
then revealed antioxidant activity by frap method: GLC5 and 2UVC8. It can be insated that
the fungi isolated from Caatinga liquens have antioxidant potential and
which indicates the possibility of biotechnological application.

BANKING MEMBERS:
Presidente - 2269479 - CAROLINNE DE SALES MARQUES
Externo(a) ao Programa - 2269720 - ANA PAULA DE ALMEIDA PORTELA DA SILVA
Externo(a) ao Programa - 2269794 - CAMILA SOUZA PORTO
Externo(a) à Instituição - ELOIZA LOPES DE LIRA
Externo(a) à Instituição - MICHEL RODRIGO ZAMBRANO PASSARINI