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THAIS RAYANE GOMES DA SILVA
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WATER CONSUMPTION OF PULSES IRRIGATED PEPPER IN DIFFERENT TIMES
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Leader : MARCIO AURELIO LINS DOS SANTOS
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MEMBRES DE LA BANQUE :
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CICERO GOMES DOS SANTOS
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GERÔNIMO FERREIRA DA SILVA
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MARCIO AURELIO LINS DOS SANTOS
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MARCOS ALEX DOS SANTOS
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Data: 16 mars 2021
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Afficher le Résumé
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Pepper (Capsicum annuum L.) is among the ten vegetables of greatest economic importance in the national market for vegetables, and to obtain high productivity it is necessary to use appropriate techniques in irrigation management. In light of this, the irrigation pulses consist of applying the necessary real irrigation in a fractional way throughout the day. Thus, the objective was to determine the crop evapotranspiration (ETc) and the cultivation coefficient (Kc) through drainage lysimeters with the inclusion of irrigation pulses at different times of cultivation in the pepper culture. The experiments were carried out in the experimental area for the Irriga Group, located on the premises of the Federal University of Alagoas, Arapiraca Campus, in the periods from January to March, May to July and August to October 2019. 25 drainage lysimeters were installed, distributed in five planting lines, in an area of 6.0 x 3.0 m in width and length, respectively, consisting of plastic material with a volume of 0.06 m3. Each set of 5 lysimeters corresponded to an irrigation pulse, the first set being called pulse 1 to set 5 which was called pulse 5. The Kc was obtained for each irrigation pulse, being determined by the ratio between ETc and the ETo (reference evapotranspiration). ETc was obtained daily for each pulse of irrigation, which was determined by the difference between the applied and drained blade. And ETo estimated through empirical methods: Penman-Monteith, Hargreaves-Samani, Solar Radiation and Blaney-Criddle. The first growing season showed the respective Kc's: pulse 1 with the lowest Kc (0.61; 0.61; 0.81; 1.09 and 1.24) and pulse 5 with the highest Kc (0.77; 0, 77; 0.89; 1.15 and 1.27), the 2nd growing season follows the same pattern of behavior with the lowest Kc for pulse 1 (0.55; 0.55; 0.74; 0.94 and 1.16) and the highest Kc at pulse 5 (0.62; 0.62; 0.76; 1.14 and 1.29), which continues to happen in the third growing season, where pulse 1 obtained lowest Kc (0.59; 0.59; 0.84; 1.04 and 1.22) and pulse 5 the highest Kc (0.70; 0.70; 1.00; 1.26 and 1.30 ) when compared to the others. In all growing seasons, ETc increased with the addition of irrigation pulses, in the 1st growing season, pulse 1 had a lower ETc with 8.36 mm (Total: 307.58 mm) and pulse 5 had a higher ETc with 8.40 mm (Total: 336.16 mm); in the second growing season, pulse 1 presented 4.41 mm ETc (Total: 171.74 mm) and pulse 5 of 4.51 mm (Total: 187.62 mm); and the third cultivation season, obtained for the wrist 1 an ETc of 8.92 mm (Total: 335.14 mm) and for the wrist 5 an ETc of 9.50 mm (Total: 386.35 mm). With respect to Kc, pulse 1 obtained the lowest and pulse 5 the highest, when compared to the others.
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2
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FRANCISMARY BARROS DA SILVA
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SEQUENCING OF CHLOROPLAST GENOMES AND PHYLOGENETIC ANALYSIS IN THE GENUS Spondias (Anacardeaceae)
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Leader : CICERO CARLOS DE SOUZA ALMEIDA
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MEMBRES DE LA BANQUE :
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CICERO CARLOS DE SOUZA ALMEIDA
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FLAVIA DE BARROS PRADO MOURA
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JOSE VIEIRA SILVA
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Data: 14 avr. 2021
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The plastome have a uniparental origin (usually maternal), while rDNA have a biparental origin, allowing to obtain robust phylogenies. The objective of this study was to assemble the chloroplast genomes of Spondias sp (umbuguela), (S. purpurea) and (S. dulcis), to build a robust phylogeny for the genus Spondias through the combination of SNP analysis, nrDNA and chloroplast alleles, using data from new generation sequencing. DNA extraction was performed using the CTAB method. The sequencing of the reads was carried out at GenOne Soluções em Biotecnologia, Rio de Janeiro, Brazil, where 5 five mg of gDNA was used for each sample to build the library. Sequencing was generated using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, UK). The library was analyzed for fragment distribution using Agilent2100 Bioanalyzer and quantification by PCR in real time. The library was of the pairedend type (2 x 150 pb) sequenced with Illumina NovaSeq 6000 sequencer. Genome assembly and SNP analysis were carried out at the Genetic Resources Laboratory at UFAL, using the (S. tuberosa) genome as and by reference the Genes annotation tool. All notes were checked manually. It was used and the complete genome was represented using the software OGDRAW (Draw Organelle Genome Maps) to represent the complete genome. For SNP analysis, the readings were mapped in the reference using the Bowtie2 software and the result due in graphical HeatMap. The species plastidial genomes of the species of the genus Spondias analyzed in the present study proved to be highly conserved, with size Spondias sp (umbuguela) (162,577 bp), (S. purpurea) (162,728 bp), and (S. dulcis) (162,245 bp) with a total of 137 genes for Spondias sp (umbuguela) and (S. dulcis), including 37 tRNAs and 8 rRNAs. While (S. purpurea) presented 136 genes, including 37 tRNAs and 8 eight rRNAs. Spondias purpurea shared a greater number of SNPs with Spondias sp (umbuguela) and are being closely related to and grouped in the same clade, suggesting what suggests that umbuguela is not a hybrid.
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3
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MARÍLIA LAYSE ALVES DA COSTA
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Characterization of humic substances from Oxisols distributed in the
state of Alagoas
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Leader : WANDER GUSTAVO BOTERO
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MEMBRES DE LA BANQUE :
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CICERO GOMES DOS SANTOS
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OSEAS SILVA SANTOS
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WANDER GUSTAVO BOTERO
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Data: 22 juil. 2021
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The semi-arid region of Northeastern Brazil (NEB) has high soil variability, highlighting the State of Alagoas, which has a strong diversity of climate, relief and soils typically belonging to the Oxisols and Argissolos classes. Studies related to Oxisols present in the state of Alagoas are still incipient, which makes it difficult to know the physical-chemical properties, as well as to provide better use of such soils, in order to avoid processes of environmental degradation, such as desertification. The present work aimed to extract and characterize the organic matter present in Yellow, Red and Red-Yellow Latosols frequently found in different regions of the state of Alagoas, seeking to establish data that can help in decision making and improvement of agriculture in areas subject to desertification. The samples of Oxisols were collected between the months of October and November of 2018, in the cities of Alagoas (Teotônio Vilela, Arapiraca, Coruripe, Palmeira dos Índios, Limoeiro de Anadia and Rio Largo). Fertility analysis of the eight samples of Oxisols consisted of determining the pH in water and calcium chloride, Calcium, Magnesium and Aluminum Method Exchangeable Calcium, Exchangeable Aluminum, Exchangeable Sodium and Potassium, and Potential Acidity The organic matter of the soil samples was determined by the calcination method, and the extraction of HS occurred by the method adopted by the International Society of Humic Substances (IHSS). The characterization of the SH samples was performed both by ultraviolet spectroscopy in the visible region and by molecular fluorescence spectroscopy. Micro,
macronutrients and metallic species were determined by the open system digestion method. Through the analysis of fertility, it was found that these are strongly acidic soils, highlighting soil H, due to the high content of expressed potential acidity. The MOS content was expressive in all samples, highlighting the E (9.23±0.49) and D (8.02±0.08) soils, which presented a higher mean MOS content when compared to the other samples, which directly influenced the increase in the cation exchange capacity. According to the E4/E6 ratio, sample B has a greater presence of condensed aromatic structures (3.98), while sample C has a greater presence of aliphatic structures (24.88), low molecular weight (11.50) and with greater participation of terrestrial plants, due to the high level of lignified structures. While the fluorescence characterization through the A4/A1 ratio, it was evidenced that the F sample has a higher humification index, where consequently there was a change in MOS in SH, expressing itself as a soil of undesirable quality. Sample E expressed a higher average content of the elements evaluated (Ba, Co, Cr, Cu, K, Mo, Ni, Pb, Sr, Zn, Al and Fe). Therefore, the present research becomes relevant to obtain a more accurate knowledge of this soil class in the state of Alagoas, as well as scientifically contributing so that these are conditioned to maintenance in order to maintain their fertility.
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4
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ADIELMA MARIA DE MENEZES
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POSTHARVEST QUALITY CHARACTERIZATION OF ACCESSES OF DIOSCOREA SPP. COLLECTED IN ALAGOAS AND SERGIPE
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Leader : JOÃO GOMES DA COSTA
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MEMBRES DE LA BANQUE :
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JOÃO GOMES DA COSTA
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JOAO PAIXAO DOS SANTOS NETO
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MAYARA ANDRADE SOUZA
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Data: 6 sept. 2021
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The aim of the study was to characterize accessions of yam Dioscorea spp. as to the proximate composition (moisture, ash, lipids, carbohydrates and proteins), the total energy value (TEV) and the content of mineral elements. The accesses were obtained from collection expeditions in the main producing municipalities in the States of Alagoas and Sergipe. Thus, two accessions of Dioscorea trifida were captured; six from D. cayenensis, one from D. bulbifera and one. D. alata. All analyzes were carried out from 11/23/2020 to 12/10/2020. The dataset was submitted to the Kolmogorov-Smirnov normality test and, as it presented normality, descriptive statistics such as mean and standard deviation and multivariate analyzes were analyzed as a determination of genetic divergence from dissimilarity measures such as the mean Euclidean distance and cluster analysis as the Tocher method. The results sought that the accessions of different species of Dioscorea have potential for use in human food and that they can be better used by local populations. As, for example, we can mention the accessions 2, 9 and 10 which have protein content above the average in relation to the other genetic materials. As well as some accessions that stood out, such as Access 10 that presented high levels of zinc, magnesium and potassium, while Access 5 stood out in relation to manganese content. On the other hand, the study showed the little variability that exists in the materials, evidencing the need to leave these materials and rescue those that the producers of the different traditional categories established to plant.
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5
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WANESKA DOS SANTOS
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Response of Carpophilus spp. to the odors of the pine tree blossom and the fruits of pine cones and guava in an advanced stage of maturation
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Leader : JOÃO GOMES DA COSTA
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MEMBRES DE LA BANQUE :
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CENIRA MONTEIRO DE CARVALHO
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EDJANE VIEIRA PIRES
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JOÃO GOMES DA COSTA
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Data: 8 sept. 2021
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Among the species belonging to the family of annonaceae, the pine cone (Annona squamosa L.), is one of the representatives of the family of greatest socioeconomic importance worldwide. In Brazil, its largest production is concentrated in the Northeast region, with family farming being responsible for all the pine cones produced in the country. However, the production of fruits of this species is below its potential due, among other factors, to deficient pollination, since normally the presence of its pollinators is insufficient to reach satisfactory productions. Artificial pollination has good results, but it is costly. Thus, the objective of this work was to use volatile organic compounds (odors) that are attractive to Carpophilus spp. both in the laboratory and in the field. The volatile organic compounds (VOCs) of the pine cone and guava fruits, as well as the VOCs emitted by the pine tree flowers, were collected using the dynamic headspace system, using Porapak Q adsorbent (80/100 mesh, 0.05 g; Supelco). The air was injected into the plastic bag, passing through an activated carbon filter at a flow of 400 mL/min. for both fruits and flowers and tubes with Porapak Q were used at the top of the bag and the air was collected at a flow rate of 350 mL/min. for each fruit/flower. The collection of VOCs from the pine cone flowers lasted twelve hours starting at 5:00 pm, as this time was marked by the release of a strong sweet odor, which remains until the next day. Previously, the glass tubes were washed with hexane and heated in ovens at 100 °C for 60 min, for the "activation" of the adsorbent. At the end of the aeration, the desorption of volatiles in Porapak Q was carried out passing 0.5 mL of hexane solvent and stored in vials at -20 °C. As verified, the volatiles of the guava fruit, the pine cone flower and the pine cone fruit showed that they are attractive to the pine tree pollinators, both in the laboratory and in the field. The VOCs of the guava fruit and the pine cone in advanced stages of maturation attracted the pine pollinating insect, as well as the flower VOCs. That's because a floral odor similar to ripe or decaying fruit can attract nidullid beetles. Since, these insects are attracted by floral scents that have a “fruitful” aroma. The flowers probably mimic fruit aromas in order to attract these insects. The use of attractive baits in pine cone orchards, aiming to increase productivity, showed satisfactory results, increasing productivity in commercial orchards by more than 80%.
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6
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MILLENA DE ARAUJO RODRIGUES
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PEANUT IRRIGATED WITH DIFFERENT WATER BLADES AND DISTINCTS DOSES OF PHOSPHATE AND ORGANIC FERTILIZATION
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Leader : MARCIO AURELIO LINS DOS SANTOS
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MEMBRES DE LA BANQUE :
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MARCIO AURELIO LINS DOS SANTOS
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CICERO GOMES DOS SANTOS
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GERÔNIMO FERREIRA DA SILVA
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MARCELO CAVALCANTE
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Data: 9 sept. 2021
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Afficher le Résumé
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Peanuts are the fourth most cultivated oilseed in the world, but in order to obtain high yields in the Semiarid region, irrigation is necessary. The production of this crop is related to soil fertility, and phosphorus is considered the main factor of crop productivity. It is also widely used as a fertilizer for chicken litter, as it presents the main nutrients (nitrogen, phosphorus and potassium). Thus, the objective was to evaluate the response of peanut crops under different irrigation depths and different doses of phosphate and organic fertilization. The experiment was conducted in the experimental area of the IRRIGA Group, at the Federal University of Alagoas, Campus de Arapiraca, with a randomized block design in a 5 x 5 x 4 factorial. The treatments applied consisted of 5 irrigation depths: 50, 75, 100, 125 and 150% of daily crop evapotranspiration (ETc), 5 doses of phosphate fertilization: 0, 50, 100, 150 and 200% of the crop recommendation, and in the other experiment, five levels of fertilization (2, 4, 6, 8 and 10 t/ha), with 4 repetitions. Each experimental plot was composed of 27 plants, distributed in 1.0 m², evaluated only the 10 central plants. The ETc was determined using the SLIMCAP system, used to apply water depths. The variables studied were: plant height (AP), number of pods (NVV) and number of stalks (NHP) per plant, average length (CMV) and average diameter (DMV) of the pod, volume of pod per plot (VVP) , green weight (PVV) and dry weight (PSV) of the pod, and relative chlorophyll index (SPAD). The results concluded were that for phosphate fertilization the variables SPAD, NVP, VVP and PVV respond to the interaction of irrigation depth and doses of phosphate fertilization. AP, NH and PS respond to lamina and phosphate fertilization alone. On the other hand, CM responds significantly only to phosphate fertilization and DM is not influenced by the factors studied. The maximum production points of most variables were obtained with the irrigation depth higher than ETc and fertilization (P2O5) lower than recommended for the crop. The results for the experiment with chicken litter showed that the irrigation depths positively influenced the variables: AP, NHP, NVP, DMV, VVP, PVV, PSV, in which they obtained better performance at 100% ETc; the variables CMV and chlorophyll index (SPAD) had a decreasing influence with the increase of irrigation depths, the greater the irrigation depth, the lower the value obtained; the doses of organic fertilizer had no positive influence, only on the chlorophyll index (SPAD), obtaining higher values in the doses closer to 100% of the recommendation.
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7
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ADRIELY VITAL DE SOUZA SILVA
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Solubilization of inorganic phosphate by filamentous fungi isolated from lichens and sediment from Antarctica
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Leader : ALYSSON WAGNER FERNANDES DUARTE
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MEMBRES DE LA BANQUE :
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ALYSSON WAGNER FERNANDES DUARTE
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FLAVIA DE BARROS PRADO MOURA
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MICHEL RODRIGO ZAMBRANO PASSARINI
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VALDEVAN ROSENDO DOS SANTOS
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Data: 9 sept. 2021
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Afficher le Résumé
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Antarctic shelter environments can different life forms adapted to physical conditions and extreme areas such as cold temperature, water availability, high UV radiation, strong winds, different pH and salinity ranges. Given this information, it is suspected that filamentous fungi adapted to such conditions develop the activity of solubilizing insoluble phosphate from the soil to release soluble phosphorus (P) and make it available to plants. In this context, the aim of this study was to isolate and identify new strains of filamentous fungi from lichens and sediments from Antarctica with the ability to solubilize inorganic phosphate. Lichens and sediments from Antarctica were collected during the OPERANTAR XXXVI expedition, in the austral summer of 2017/18. The 6 lichens of different colors were collected in King George Island - Punta Ullmann and Punta Hanequin, and the 4 from sediments collected in Deception Island, Whalers Bay, due to the South Shetlands (Maritime Antarctica). A collection of 82 fungal strains were selected and evaluated for an inorganic phosphate solubilization activity. Make the total of 82 fungi tested, 3 lichen and 8 sediment grant, dissipation activity for phosphate solubilization in solid NBRIP culture medium containing tricalcium phosphate (TCP) at 15 °C. 6 isolates were selected during screening in solid medium with larger solubilization halos and tested in temperature ranges, varying between 15.0, 25.0 and 30.0 °C. In solid medium, the fungi were able to solubilize in the three temperature ranges, with a solubilization index (IS) ranging from 1.4 to 3.63 mm subjected to a temperature of 15 °C, from 1.3 to 3.57 mm to 25 °C and from 1.3 to 1.4 mm at 30 °C. One of the largest IS was found in isolates 1EM.P1 at 15 °C and 5Y.P4 at 25 °C with 3.63 and 3.57 mm, respectively. In a liquid medium, the 1EM.P1 isolate was the only one to present significant values for solubilization, with an average release of soluble P of 106.14 mg/L-1 and with a pH reduction from 7.0 to 4.38 to 15 °C, and at 25 °C with soluble P release of 80.89 mg/L-1 and pH reduction from 7.0 to 5.40. For tests under stress conditions, the 1EM.P1 isolate showed better solubilization when subjected to 0.5 M NaCl. Regarding the pH variation, it showed greater release of soluble P at pH 8.0. As for the carbohydrate source, it presented better solubilization in the presence of glucose. The 1EM.P1 isolate, when subjected to different sources of inorganic phosphate, showed better solubilization in the presence of TCP, with a maximum peak in 15 days of incubation. In general, the results showed that Antarctic fungi are able to solubilize phosphate in solid medium, as well as through the release of soluble P in the liquid medium, especially those isolated from sediment.
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8
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JARDIELSON DA SILVA SANTOS
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EVALUATION OF THE PRODUCTIVITY OF IRRIGATED LETTUCE UNDER PROTECTED CULTIVATION IN RESPONSE TO THE WATER TREATMENT STATION SLUDGE APPLICATION
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Leader : WANDER GUSTAVO BOTERO
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MEMBRES DE LA BANQUE :
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ANDREA PIRES FERNANDES
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MARCIO AURELIO LINS DOS SANTOS
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WANDER GUSTAVO BOTERO
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Data: 10 sept. 2021
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Afficher le Résumé
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Water for human consumption must meet a potability standard in order to be used for drinking, food preparation and personal hygiene. For this, it is necessary that, regardless of its origin, the water is submitted to different physical and chemical treatments, or a combination of both. Water treatment consists basically in the removal of suspended and colloidal particles, organic matter, microorganisms, and other substances possibly harmful to human health. After the removal of particles a residue called sludge is generated, which is a mixture of sand and clay, rich in organic matter (OM), micro and macro nutrients. This waste has still been disposed in water courses without any previous treatment. For its reuse is of fundamental importance the study of the structural characteristics, as well as the evaluation of the OM content present in this waste. Thus, the objective of this study was to extract and characterize the OM of the sludge from the Water Treatment Plant in the city of Arapiraca, Alagoas, and then apply it to the lettuce crop in order to evaluate the influence of OM on plant growth and development. The samples were collected and submitted to drying and then disaggregated and sieved. The results obtained in this study indicate that this waste is rich in OM, indicating that this matrix has a potential as an agricultural additive. The E4/E6 ratio shows that the humic substances (HS) contain few aromatic structures and a lower degree of humification. The E2/E3 ratio showed a high value, indicating a decrease in the molecular size of the structures that compose the HS. From the E2/E4 ratio it can be inferred that in the studied SH, there is the presence of structures derived from lignin. The HS, contributed to the growth and development of the lettuce plants. For most of the variables analyzed, the HS showed a significant effect. The HS dose of 100 mg L-1 was the best response dose. A decrease in the effects of the analyzed variables was observed as the concentration of HS increased.
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9
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RAFAELA FERREIRA DA SILVA COSTA
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SOLUBILIZATION OF INORGANIC PHOSPHATE BY BACTERIA ISOLATED FROM ANTARCTICA LICHEN
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Leader : ALYSSON WAGNER FERNANDES DUARTE
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MEMBRES DE LA BANQUE :
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ALYSSON WAGNER FERNANDES DUARTE
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JOÃO GOMES DA COSTA
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MICHEL RODRIGO ZAMBRANO PASSARINI
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VALDEVAN ROSENDO DOS SANTOS
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Data: 10 sept. 2021
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Afficher le Résumé
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Lichens are pioneer organisms in extreme environments, grow in nutrient-limiting conditions and are recurrent in Antarctica. They represent a rich reservoir for the isolation of bacteria with biotechnological potential of agricultural interest, presenting characteristics that promote plant growth through inorganic phosphate solubilization mechanisms. In this context, the present study aimed to analyze the potential of bacteria isolated from 6 Antarctic lichens for solubilization of inorganic phosphate under the influence of different temperatures, pH variation, NaCl concentrations, carbohydrates and induction of osmotic potential. The collection took place from different lichens on King George Island, during the OPERANTAR XXXVI expedition (summer 2017/2018). Bacteria were tested for phosphate solubilization using solid NBRIP medium (qualitative) and in broth (quantitative). The positive bacteria for solubilization were tested for the ability to fix nitrogen. The quantification of soluble phosphorus in the supernatant of the isolates was estimated by the ammonium vanadate-molybdate method. Of 60 bacteria tested, 15 (25.0%) solubilized tricalcium phosphate at 15.0 ºC. At temperatures of 15.0 ºC (15= 100%) and 25.0 ºC (15= 100%) all strains solubilized phosphate in solid culture medium, whereas at 30.0 ºC 13 strains (13= 87. 5%) and at 35.0ºC there was no growth of any isolate. The enzyme index for phosphate solubilization was measured by the ratio of the diameter of the halo divided by the diameter of the colony. The highest phosphate solubilization indexes at 15.0 ºC were from 1.75 mm to 3.02; at 25.0 °C from 1.84 mm to 2.76 mm and at 30.0 °C from 1.78 mm to 2.70 mm. The best bacteria at the three temperatures were 1B.LQ4; 4BLQ4; 4BLQ4* and 2BLQ1*, together with 7BLQ2 (randomly chosen) were selected for phosphorus quantification in liquid medium at 25.0 ºC ± 2.0 ºC. Strains 1BLQ4 and 4BLQ4 had an average of 605.18 mg/L and reduced the pH to 4.07, while 2BLQ1* solubilized 505.56 mg/L and final pH of 4.29 and 4BLQ4* with 494.05 mg /L and pH 4.17. These four isolated showed no significant difference in soluble P. The lowest mean of soluble P was from strain 7BLQ2 with 365.78 mg/L and pH from 7.0 to 4.33. Of the 15 phosphate solubilizers, 80% (13) grew in selective medium for nitrogen fixatives and the strains that showed the greatest growth were 2BLQ1* and 3BLQ5. Bacteria 1BLQ4 and 2BLQ1* were selected for testing under stress conditions. Both bacteria solubilized phosphate at the concentration of 0.5 M Nacl with averages of 421.81 mg/L and 432.77 mg/L, respectively. At the different pH tested (7, 8 and 9), the two strains continued with high levels of soluble P. When subjected to tests with different carbohydrates (glucose, sucrose, lactose and starch), glucose was the most efficient for the solubilization mechanism of the two isolates. 2% glycerol was used; 5% and 10% to induce osmotic potential and both strains maintained high levels of soluble P in the 3 conditions tested. Assessing the maximum point of bacterial solubilization every 24 hours for 6 consecutive days, the best result for 1BLQ4 was with 96 h of incubation (606.45 mg/L) and for 2BLQ1 * it was with 120 h (565.41 mg/L) L). The results demonstrate that these strains are efficient in the solubilization mechanism of inorganic phosphate at different temperatures, alkaline conditions, osmotic effect and reasonable concentrations of salinity.
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10
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LARYSSA ROBERTA ALVES FARIAS
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AGRONOMIC PERFORMANCE OF PEANUT CULTURE IRRIGATED WITH SALINE WATER IN A PROTECTED ENVIRONMENT
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Leader : MARCIO AURELIO LINS DOS SANTOS
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MEMBRES DE LA BANQUE :
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MARCIO AURELIO LINS DOS SANTOS
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INAJÁ FRANCISCO DE SOUSA
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DANIELLA PEREIRA DOS SANTOS
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SAMUEL SILVA
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Data: 11 sept. 2021
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Afficher le Résumé
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The implementation of areas with irrigated peanuts in the region is still limited due to water availability and little knowledge of this technique for this culture in the region. The implementation of irrigation in the crop allows the cultivation of two or more peanut cycles during the year, thus increasing the income of small farmers who are the main producers. The objective of this research was to evaluate the agronomic performance of peanuts in a protected environment, under the effect of different water depths and different levels of water quality. The experiment was carried out in a greenhouse in the experimental area of the Arapiraca Campus at UFAL. The experimental design was randomized blocks (DBC), with 4 replications, in a 5x5 factorial scheme, with 25 treatments, totaling 100 plots. The treatments represented five irrigation depths L1=50; L2=75; L3=100; L4= 125 and L5= 150% of crop Evapotranspiration correlated with saline water levels represented by S1 = 0.15; S2 = 1.15; S3 = 2.15; S4 = 3.15 and S5 = 4.15 dS/m. The determination of ETc was obtained using SLIMCAP. The method of localized irrigation with a drip system with an average flow of 2.5 L/h per dripper was used. The variables were evaluated: plant height (AP), number of stalks per plant (NHP), number of pods per plant (NVP), average pod length (CMV), average pod diameter (DMV), number of grains per pod (NGV), pod fresh weight (PFV) and pod dry weight (PSV), chlorophyll index (SPAD). The collected data were subjected to statistical analysis and surface graph plots using the R i386 statistical software. The results showed that the variables plant height index (AP), number of pods per plant (NVP), average length of pods (CMV) and chlorophyll index (SPAD) respond to the interaction of irrigation depth and water salinity levels . The number of stalks per plant (NHP), mean diameter of pods (DMV), volume of pods per plant (VVP) and green weight of pods (PVG), dry weight of pods (PSG) respond to lamina and salinity separately. The number of grains per pod responds significantly only to water salinity. The maximum production points for most variables were obtained with irrigation depths below 100% of ETc and salinity close to 0.15 dS/m.
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11
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MARIA APARECIDA DOS SANTOS
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ASSEMBLY, ANNOTATION OF PLASTIDIAL AND MITOCHONDRIAL GENOMES OF Stylosanthes seabrana and Stylosanthes guianensis
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Leader : ANDRE SECO MARQUES DA SILVA
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MEMBRES DE LA BANQUE :
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ANDRE SECO MARQUES DA SILVA
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CICERO CARLOS DE SOUZA ALMEIDA
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LUIS GUSTAVO RODRIGUES SOUZA
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Data: 16 sept. 2021
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The genus Stylosanthes Sw. is highly diverse and polymorphic, many species are present in regions of low rainfall, grow in calcium-poor soils, are able to fix nitrogen in the soil, and are of great importance in the formation of pastures and also in the recovery of degraded areas. Despite its economic importance, the genus Stylosanthes still presents little genetic characterization and its taxonomy and systematic complexity makes the delimitation and identification of species difficult. Organellar genomes, such as those of chloroplast and mitochondria, can be very useful in resolving phylogenetic relationships in groups with complex systematics. Due to their uniparental, mainly maternal inheritance in angiosperms. The objective of this work was to obtain and analyze comparatively the complete Plastome and Mitogenome of two species of the genus Stylosanthes: S. seabrana, S. guianensis in order to increase the knowledge about the evolutionary relationships and genetic improvement of the genus. The genomes of each species were assembled using NOVOPlasty v. 3.8.3 software, using as reference the plastidial and mitochondrial genome of Stylosanthes hamata. After assembly, with the help of Geneious v. 9.1.8 software, the genomes were analyzed and compared. The circular plastidial genome for Stylosanthes seabrana showed a total size of 156,499 bp, and a total of 125 genes were identified. In turn, the plastidial genome of Stylosanthes guianensis showed a total size of 156,785 bp, being identified a total of 127 genes. The mitochondrial circular genomes obtained for the two species presented a length of 468,896 bp in S. guianensis, being identified 55 genes and in S.seabrana of 523,870 bp, being identified 57 genes. The plastid and mitochondrial genomes of the species of the genus Stylosanthes analyzed in this study proved to be highly conserved and can be used for further phylogenetic studies and contribute to the genetic improvement programs of these species.
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12
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FERNANDA DANIELLE DE SOUSA
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The complete mitochondrial genome of the Hancornia speciosa Gomes and evolutive analysis in Apocynaceae
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Leader : CICERO CARLOS DE SOUZA ALMEIDA
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MEMBRES DE LA BANQUE :
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CICERO CARLOS DE SOUZA ALMEIDA
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ANDRE SECO MARQUES DA SILVA
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LUIS GUSTAVO RODRIGUES SOUZA
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Data: 17 sept. 2021
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Hancornia is a monotypic genus that belongs to the Apocynaceae family, its only species is an H. speciosa Gomes, also known as mangaba. It is a fruit tree, native to Brazil, appreciated for its sweet and slightly acidic flavor that makes it quite characteristic. In addition to its ecological and industrial importance, it is a source of income for agro-extractivists. The mitochondrial genome of plants has important evolutionary characteristics, such as the large size of intergenic spacers and a large number of rearrangements, and has been studied in many groups of plants. In order to understand the phylogenetic relationships, evolutionary aspects and contribute to research on plant breeding, the mitochondrial genome of Hancornia speciosa was sequenced by Next Generation Sequencing (NGS). And through the de novo assembly and union of contigs, the complete mitochondrial genome was obtained with a size of 741,811 bp in length and 63 annotated genes, becoming the longest reported in Apocynaceae. In summary, we demonstrate: (1) the first mitochondrial genome of Hancornia speciosa; (2) The incorporation of chloroplast DNA into the mitochondrial genome in Hancornia speciosa is smaller than Rhazya stricta, Hoya lithophytica and Asclepias syriaca and its expansion may be associated with recombination events and increase in the intergenic region; (3) The synteny between H. speciosa and Rhazya stricta, which belong to the Rauvolfioideae subfamily, and between Hoya lithophytica and Asclepias syriaca, which belong to the Asclepiadoideae subfamily, show remarkable rearrangements; (4) The association of data obtained between mitochondrial rearrangements and plastid nucleotide sequences reveals that the closer genomes share more regions in common. Comparative analyzes show the remarkable diversity of the mitochondrial genome already reported in angiosperms, which shows high conservation of genes in their functioning and high structural plasticity.
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13
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NATANIELLE DE OLIVEIRA GOMES LEITE
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BIOSPECKLE TECHNIQUE APPLICATION IN THE MORPHO-PHYSIOLOGICAL CHARACTERIZATION OF AUREA MART Tabebuia leaves. IN TWO LIGHT AND WATER CONDITIONS
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Leader : JOSE VIEIRA SILVA
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MEMBRES DE LA BANQUE :
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EMERSON DE LIMA
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JAKSON LEITE
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JOSE VIEIRA SILVA
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RUBENS PESSOA DE BARROS
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Data: 20 sept. 2021
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The biospeckle technique has been used as a tool for analyzing the biological materials activity, monitoring moisture, activity level and presence of parasites, through of the material illumination by a low power laser source. As it is a non-destructive technique, the biospeckle is an advantageous tool in fast procedures and with great precision, especially in the biological systems analysis. Thus, the objective of this research was to identify and characterize the leaf morphophysiology of Tabebuia aurea (Mart.) species submitted to water restrictions and the application of a particle film using biospeckle technique. The experiment was conducted at the Plant Physiology (LABFIVE) and Biospeckle (LEB) Laboratories and in a greenhouse at the Federal University of Alagoas, Campus Arapiraca. The experimental design was in randomized blocks (DBC) with 4 treatments [irrigated + presence of film; irrigated + absence of film; non-irrigated + film presence; non-irrigated + no film] with 4 repetitions for each treatment. Tabebuia aurea (Mart.) seeds collected from matrix trees located on the Campus Arapiraca of the Federal University of Alagoas - UFAL, were germinated in polyethylene pots with a capacity of 3L in a greenhouse (with shading of ~ 50%) until establishment of the seedlings. After the growth and seedlings establishment, the plants were transferred in the early morning hours (between 5:30 and 6:00 a.m.) to the environment outside the greenhouse under full sun, and subjected to treatments. After the treatments imposition, the evaluations were carried out for a period of 30 days. In which, the morphological and biological activity analyzes in the leaves were started at time 0, 24 hours (one day), 48 hours (two days), 72 hours (three days), seven days, 15 days and 30 days. The collected data were submitted to statistical analysis and graph plotting using the R i386 statistical software. The variables plant height index (AP), stem diameter (mm), and number of leaves in Tabebuia aurea (Mart.) plants, as well as biological activity (MDI) and homogeneity responded significantly between treatments irrigation (with and without) in the presence or not of the particulate film.
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14
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Fabiana Fontes Silva Macedo de Carvalho
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NUTRITIONAL CHARACTERISTICS AND FORAGE PALM BIOMASS ACCUMULATION (Nopalea cochenilifera (L.) Salm-Dyck) INFLUENCED BY STYLOSANTHES AND ORGANIC FERTILIZATION.
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Leader : CICERO GOMES DOS SANTOS
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MEMBRES DE LA BANQUE :
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CICERO GOMES DOS SANTOS
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MARCIO AURELIO LINS DOS SANTOS
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KEDES PAULO PEREIRA
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CAROLINA CORRÊA DE FIGUEIREDO MONTEIRO
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Data: 24 sept. 2021
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The forage cactus (Nopalea cochenillifera (L.) Salm – Dick) has morphophysiological characteristics that allow its development in conditions of low water supply, and has played an important role in livestock in the Semi-arid region, forming the basis of ruminant feeding. The production of forage cactus in the Northeast of Brazil presents low productivity mainly due to inadequate management, lack of correction of nutritional levels and low levels of organic matter in the soil. The use of Stylosanthes species as green manure can favor the production system of forage cactus, working as an alternative source of nutrients, especially nitrogen, to inorganic fertilizers. The contribution of plant residues to the soil favors the increase in organic matter content, nutrient cycling, microorganism activity, structuring, infiltration and storage of water in the soil, improving the quality and resilience of agroecosystems. This work aimed to evaluate the nutritional composition and biomass accumulation of forage cactus cultivated in the residue of stylosanthes cv. Campo Grande as green manure and organic manure using cattle manure. The experiment was carried out in the experimental area of the Federal University of Alagoas - UFAL, Campus Arapiraca, in randomized blocks, with four replications, in a 3x2 factorial scheme. The first factor studied corresponded to fertilization: a) green fertilization with stylosanthes cv. Campo Grande associated with chemical phosphate fertilization, b) organic fertilization with cattle manure associated with chemical phosphate fertilization, and c) control with chemical phosphate fertilization without green and organic fertilization. The second factor studied was spacing: 1.20 x 0.20 and 1.20 x 0.25, with plant density per area of 41,000 and 33,000 plants per hectare, respectively. Biometric variables were evaluated: plant height (AP), plant width (LP) and number of cladodes emitted per plant (NC), starting at 180 days after planting (DAP), with an interval of 60 days between measurements; chemical-bromatological and mineral composition; chlorophyll and carotenoid contents in two distinct periods; and production of green and dry biomass in 12 months. To perform the statistical analysis, the SISVAR software, version 5.6 was used. The variables studied show significant differences in relation to treatments with green and organic fertilizers, and significant biomass production with organic fertilizer and 1.20 x 0.20 spacing in relation to the other treatments
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15
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JÂNIO PEDRO DA SILVA VIEIRA
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BIOMASS, DECOMPOSITION AND NUTRIENT RELEASE OF SHOOT COVER CROPS IN THE AGRESTE REGION, ALAGOAS, BRAZIL
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Leader : VALDEVAN ROSENDO DOS SANTOS
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MEMBRES DE LA BANQUE :
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VALDEVAN ROSENDO DOS SANTOS
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GILSON MOURA FILHO
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RUBENS PESSOA DE BARROS
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Data: 27 sept. 2021
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Green manure consists in the cultivation of different plant species in the same area, in succession or simultaneously, with the main purpose of improving soil fertility. Thus, it is preferred that those that produce a greater volume of dry matter, are less subject to pests and diseases and that have relatively uniform and easy to sow seeds. The Fabaceae family plants are the most used in green manure, due to their high potential to carry out the fixation of atmospheric nitrogen through symbiosis with bacteria present in nodules located in plant roots, and their growing capacity to store biomass and nutrients, characterizing itself as one of the most suitable methods for use in agriculture. The aim of the present study was to evaluate biomass production, decomposition rates and nutrient release dynamics from the aboveground part of cover crops in the Agreste region, Alagoas, Brazil. The experiment was carried out under field conditions, in the experimental area of the Federal University of Alagoas - Campus Arapiraca, in randomized blocks, with four replications. The treatments consisted of cover crops: sunn hemp (Crotalaria juncea), Spectabilis (Crotalaria spectabilis), dwarf pigeon pea (Cajanus cajan), lablab (Dolichos lablab), jack bean (Canavalia ensiformis), millet (Pennisetum glaucum) and black velvet bean (Mucuna aterrima). The decomposition and release of nutrients from the residues was monitored through periodic collections at 30, 60, 90, 120 and 150 days after the incorporation of litter bags in the soil containing 20 grams of dry mass. Biomass production, extraction and nutrient use efficiency, and the decomposition and release of nutrients from waste were evaluated. The studied variables show significant differences in relation to nutrient concentrations, C/N and C/P ratio in dry mass, yield, extraction and nutrient use efficiency by the aerial part of cover crops in full bloom. Decomposition rates and half-life times varied with the cover crop species, with jack bean being the species with the highest rates and half-life times.
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16
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ALEYRES BISPO CHAGAS
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Study of biological activity in Enterolobium Contortisiliquum (Vell.) Morong seeds using the biospeckle technique
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Leader : JOSE VIEIRA SILVA
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MEMBRES DE LA BANQUE :
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EMERSON DE LIMA
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FLAVIA DE BARROS PRADO MOURA
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JOSE VIEIRA SILVA
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RUBENS PESSOA DE BARROS
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Data: 28 sept. 2021
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The biospeckle laser technique is based on an optical interference phenomenon that happens when a coherent light beam falls on the material surface, where there is activity of biological origin. Thus, the objective of this work was to characterize the metabolic activity through the biospeckle technique, correlating it with the seed imbibition curve after chemical and mechanical scarification and at different times. For this, newly collected E. contortisiliquum seeds were subjected to five different treatments, namely Control, H2SO4(PA) at 5 minutes, H2SO4(PA) at 10 minutes, H2SO4(PA) at 15 minutes and Mechanical Scarification, and exposed at soak times of 0, 8, and 72 hours. The experiment was carried out in a completely randomized design and all treatments consisted of five replicates consisting of 100 seeds each. The seeds were weighed on an analytical balance with a precision of 0.001 g before and after imbibition and an imbibition curve was established. For the analysis of the metabolic activity of seeds by the biospeckle technique, newly collected E. contortisiliquum seeds were used and submitted to the same five treatments described above, and soaking time of 0, 4 and 72 hours, with five replicates of 57 seeds. The TCAPTURE, COMMAND PROMPT, STDM programs were used, and among the lines that form the Y axis of the matrix, the line 100 was established as the standard line for the study of changes occurring in a region of the sample surface. The analysis of the speckle patterns temporal evolution was performed using a THSP image, followed by the calculation of the MCO. Chemical scarification treatments with concentrated sulfuric acid proved to be efficient in overcoming seed tegumentary dormancy, since increasing imbibition occurred with times of 0, 8 and 72 hours. The seeds imbibition resulted in the primary root emission and integument disruption at the base of the seed, close to the hilum; the primary root showed a pivoting shape, light yellow in color and with few simple hairs. Soon after, from 48 to 72 hours, the tegument detachment occurred, leaving the fleshy cotyledons free, characterizing a phanerocotyledonary epigeal germination, with the control treatment exception. The hypocotyl is elongated and straight, with a light grayish-green coloration. The epicotyl is grayish-green yellow in color. The eophylls are composed, opposite, bipinnate, and have a dark yellow grayish-green color. The development of uniform seedlings, with the control treatment exception, which had little water absorption by the seeds. They are also evaluated using the biospeckle technique where the calculated descriptors are defined and mathematically formalized, by measuring the homogeneity or uniformity of the texture. This results in higher values when the intensities are uniform and the Inertia (MI) was more efficient in the analysis of high frequencies, which is due to its actuation mechanism, which is a second order moment, which favors variations in highest activity.
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17
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JANYNNE JOYCE DE LIMA ROCHA
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Changes in physical and chemical soil attributes as a function of land use in the municipality of Limoeiro de Anadia - AL
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Leader : CARLOS TADEU DOS SANTOS DIAS
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MEMBRES DE LA BANQUE :
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CARLOS TADEU DOS SANTOS DIAS
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VALDEVAN ROSENDO DOS SANTOS
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GILSON MOURA FILHO
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RUBENS PESSOA DE BARROS
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Data: 29 oct. 2021
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The study of changes in the physical and chemical attributes of the soil allows establishing parameters capable of assessing soil quality. The objective of this work was to test the hypothesis that the intensification of agriculture through the cultivation of sugarcane, corn and pasture alters physical and chemical properties of the soil, when compared to land use with native vegetation. Soil samples were collected in four areas (1 hectare each, with 12 sampling points) in three layers (0.0 – 0.10; 0.10 – 0.20; 0.20 – 0.30 m ) in the municipality of Anadia Lemon Tree (Alagoas State) and analyzed for acidity and CTC properties (pH, CTCpH7, base saturation, aluminum saturation, Al+H and Na), macronutrients (P, K, Ca and Mg) and attributes physical (soil texture, soil density, particle density, total porosity, soil mechanical resistance to penetration and aggregate stability). Data were analyzed using statistical programs.
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