Banca de DEFESA: EDER DA SILVA ROCHA SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : EDER DA SILVA ROCHA SANTOS
DATE: 16/08/2023
TIME: 14:00
LOCAL: famed
TITLE:

Evaluation of leishmanicide activity of microorganism extracts isolated from the Caatinga biome.

KEY WORDS:

Microbial extracts, leishmanicide activity, Caatinga.

PAGES: 35
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Leishmaniasis is anthropozoonosis caused by protozoa of the genus Leishmania
sp. and transmitted by vectors, mainly affect less-favored populations, where the
access, medical care and drug therapy are limited, causing serious
public health problems. For these reasons too,
and are endemic in several countries. Clinical forms are varied, from
simple skin lesions that may spontaneously disappear into the visceral form,
affecting several organs, and may lead the individual to death. In Brazil, for all
forms of leishmaniasis, first-line treatment is pentavalent antimonials
Sb(V), currently the available antimonials are N-methyl-glucamina antimoniate
(Glucantime) and sodium sybogluconate (Pentostan) for all forms of leishmaniasis,
the first-line treatment are pentavalent antimonial Sb(V), currently the
antimonial scans are N-methyl-glucamina antimoniate (Glucantime) and
sodium sybogluconate (Pentostan), in addition to other second-choice drugs, however,
cause a number of side effects and resistance on the part of patients. In view of this, the
bioprospecting of natural products is an excellent alternative for
production of new antiparasitium drugs, especially bioactive compounds of
microorganisms, including bacterial ones. To this end, the present study aims to
investigate the leishmanicide activity of extracts of microorganisms isolated from the biome
Caatinga. To this do so, five bacterial isolates obtained from liquen samples were
nutrient broth for seven days at 25 °C for biomass production. The
intracellular pigmented metabolites were extracted with methanol and ethyl acetate,
the extractive solutions obtained were dried in a vacuum desiccator.
Subsequently, the cytotoxic activity against macrophages of the J774 scan was evaluated. A1
using the MTT method. The anti-promastigote activity of
Leishmania amazonensis and Leishmania chagasi, after cultivation of the parasite with the products
Natural. It was found that the extracts did not present cytotoxic effect for macrophages.

BANKING MEMBERS:
Presidente - 2272670 - ALINE CAVALCANTI DE QUEIROZ
Externo(a) à Instituição - AMANDA EVELYN DA SILVA
Interno(a) - 2269479 - CAROLINNE DE SALES MARQUES
Externo(a) à Instituição - FLAVIANA SANTOS WANDERLEY
Interno(a) - 3621153 - JULIANA CELIA DE FARIAS SANTOS
Interno(a) - 2370894 - MICHELLE JACINTHA CAVALCANTE OLIVEIRA