Use of Infrared Thermography to detect mastitis in crossbred of Murrah × Jafarabadi female buffaloes (Bubalus bubalis)
Bubalus bubalis, somatic cell count, california mastitis test, milk production, thermal images.
Mastitis is an inflammation of the mammary gland of multifactorial origin, and one of the
main diseases of dairy cattle that cause high economic losses. The monitoring, prevention
and diagnosis of some diseases can demand time, supplies, sample collection, transport,
laboratory analysis and skilled labor. These activities increase the cost of production and, in
some cases, the use of invasive techniques and/or animal containment is necessary, which
can cause stress. The objective of the present research was to evaluate the non-invasive
detection capacity of clinical and subclinical mastitis by infrared thermography (IRT) in
superficial areas of the udder associated with black background mug (BBM), somatic cell
count (CCS) and california mastitis test (CMT) in female crossbred dairy buffaloes (Murrah
x Jafarabadi). The experiment is in progress, being used 30 female buffaloes from a farm
located on the State of Alagoas. Three diagnostic tests for mastitis have been performed:
BBM, CCS and CMT. The experimental design is CRD (completely randomized design)
with 2 treatments and three replications: treatment 1: control group (thermal images of
females negative for clinical or subclinical mastitis) and treatment 2: positive group (positive
females for clinical or subclinical mastitis). Thermographic images are obtained from the
superficial regions of the udder: right posterior quarter (RPQ) and posterior left quarter
(PLQ). Collections of climate data, thermal images and milk samples are carried out every
60 days before morning milking. To obtain thermographic images, a thermographic camera
model Flir tg165® has been used. For temperatures record, the camera was calibrated to an
emissivity of 0.98, ideal for thermographic studies of biological materials, and images taken
at a distance of approximately 0.50 meter from the anatomical region studied. For data
analysis, multivariate analysis of principal components (ACP) and canonical correlation
(CCA) will be performed, with the aim of evaluating the relationship between the infrared
thermal variables with the female buffaloes mastitis tests, identifying the possible sensitivity
of IRT in detecting the mastitis in the present production system.