Banca de QUALIFICAÇÃO: MARILENE BRANDÃO TENÓRIO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARILENE BRANDÃO TENÓRIO
DATE: 16/12/2021
TIME: 08:30
LOCAL: meet.google.com/ntv-cbgt-rbr
TITLE:

CHARACTERIZATION OF INFLAMMATORY AND REDOX IMBALANCE BIOMARKERS IN UMBILICAL CORDS FROM PREGNANCIES WITH PREECLAMPSIA

KEY WORDS:

Pregnancy; oxidative stress; antioxidant

PAGES: 184
BIG AREA: Outra
AREA: Tecnologia e Inovação
SUMMARY:

Introduction: The umbilical cord is responsible for connecting the developing fetus and the placenta, with an essential role for adequate fetal growth and development. Therefore, morphological and functional changes in this can lead to diseases, such as preeclampsia (PE), and bring adverse outcomes to pregnancies. Objective: To compare the levels of redox imbalance and inflammation biomarkers in pregnancies with PE and without PE and analyze their relationship with perinatal outcomes. Methods: Cross-sectional study, carried out in Maceió-AL, in 2017, including women diagnosed with PE and others without the disease, through the application of a standardized questionnaire containing socioeconomic and clinical data. After delivery, an umbilical cord sample was collected to measure the levels of the following biomarkers: superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GR), malondialdehyde (MDA), hydrogen peroxide (H2O2), interleukin (IL) 6, 8 and 10, tumor necrosis factor-alpha (TNF-α) and myeloperoxidase (MPO). Furthermore, data on newborns from hospital records were collected to assess the outcomes of pregnancies. Statistical analyzes were performed using Stata software version 13.0 and IBM Statistical Package for Social Sciences (SPSS) version 20.0, adopting a confidence level of 95% (α=0.05), and the chi-square test, Wilcoxon–Mann –Whitney and multinomial and Poisson regressions. Results: 150 pregnant women were studied, 100 with PE and 50 without the disease. The levels of GSH (p ≤ 0.001), GPx (p = 0.016), and MDA (p = 0.028) were higher, as well as IL-6 (p = 0:030) and TNF-α (p ≤ 0:001) were lower in the PE group compared to those without the disease, in addition to their association with perinatal outcomes, including low birth weight, newborns small for gestational age, low birth length and Apgar score in the 1st and 5th minutes of life, being the interaction between groups not significant. Conclusion: Increased levels of GSH, GPx, and MDA and reduced levels of IL-6 and TNF-α demonstrate the presence of redox imbalance in umbilical cords of pregnancies with PE and the presence of an adaptive mechanism aimed at fetal protection. Therefore, it is necessary to carry out more studies on the role of the cord in PE and clinical trials using antioxidant supplementation in this group to analyze their effects on the umbilical cord and the outcomes of pregnancies affected by this illness.

BANKING MEMBERS:
Externa ao Programa - 1851685 - ALANE CABRAL MENEZES DE OLIVEIRA
Externa ao Programa - 2022362 - ANA CATARINA REZENDE LEITE
Externa ao Programa - 4364569 - FABIANA ANDREA MOURA
Interna - 1105255 - JERUSA MARIA DE OLIVEIRA AMORIM
Interno - 1653558 - LUCIANO APARECIDO MEIRELES GRILLO
Interna - 1358530 - MAGNA SUZANA ALEXANDRE MOREIRA
Presidente - 2120103 - MARILIA OLIVEIRA FONSECA GOULART
Externa ao Programa - 1120995 - SANDRA MARY LIMA VASCONCELOS