Banca de DEFESA: BRENO ARAÚJO DE MELO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : BRENO ARAÚJO DE MELO
DATE: 27/10/2022
TIME: 13:30
LOCAL: Auditório do Renorbio - NPM/UFAL (Severinão Campus A. C. Simões)
TITLE:

Use of Infrared Thermography to detect mastitis in crossbred of Murrah × Jafarabadi female buffaloes (Bubalus bubalis)

KEY WORDS:

Bubalus bubalis, Somatic cell count, California mastitis test, milk production, thermal images.

PAGES: 123
BIG AREA: Multidisciplinar
AREA: Biotecnologia
SUBÁREA: Biotecnologia em Agropecuária
SUMMARY:

Mastitis is inflammation of the mammary gland of multifactorial origin. It is mainly caused by bacteria and causes economic losses for the entire dairy production sector. The control and diagnosis of mastitis on farms can be costly and time-consuming, demanding laboratory analysis. Diagnostic imaging by infrared thermography (IRT) has become promising in veterinary medicine, as it is a non-invasive method and does not require animal restraint, being able to detect body temperature variations associated with inflammatory processes, such as fever and physiological changes. The objective of the present research was to evaluate the efficiency of IVT for the detection of subclinical mastitis in dairy buffaloes, making the association between the infrared images of the udder surface and the results of the conventional tests for the diagnosis of mastitis: Somatic cell count (SCC) and California mastitis test (CMT). 300 mammary quarters of crossbred dairy buffaloes (Murrah × Jafarabadi) were evaluated. Thermographic images were obtained from the following regions of the udder: right hindquarter (RQ) and left hindquarter (LQ). Thermal imaging and milk samples were taken at 60-day intervals between the years 2021 and 2022. Samples were obtained before the morning milking. To obtain the thermographic images, a thermographic camera model Flir tg165® was used. The camera was calibrated for an emissivity of 0.98. The images were collected with an angle of 90° and a distance of 0.50 meters. The diagnosis of subclinical mastitis was performed by CMT and CCS. The results for CMT were based on a score where: 0 = negative reaction, 1(+) = moderately positive reaction, 2(++) = positive reaction and 3(+++) = strongly positive reaction. For the CCS, samples of 50 mL of milk were collected from each breast room studied and then sent to the Clínica do Leite – Piracicaba/SP. Animals with CCS values ≤ 200,000 cells/mL were considered healthy, and those with CCS >200,000 cells/mL were diagnosed with subclinical mastitis. Correlation analyzes were performed Pearson (at 5%) and Linear regression between CCS, CMT and temperature. Sensitivity and specificity analyze were performed to determine the ability of TIV to detect subclinical mastitis in dairy buffaloes. The CMT and CCS tests identified that of the 243 mammary quarters evaluated, 61.72% (150/243) were healthy buffaloes and 38.27% (93/243) had subclinical mastitis. The CCS of healthy breast quarters ranged from 9.00 to 136.00 (×103 cell/mL), while that for diseased breast quarters ranged from 201.00 to 7411.00 (×103 cell/mL). Score 3(+++) presented the highest mean value for CCS (2839.48 ×103 cell/mL). The TIVU of scores 0; 1(+); 2(+) and 3(+++) differed significantly, ranging from 33.52°C to 35.8°C. Score 3+ had the highest mean TIVU value (34.83°C). The CMT identified that the highest proportion of breast rooms evaluated were classified with a score of 1+ (40.00%), followed by scores of 3+ (35.48%) and a score of 2++ (24.73%). There were significant positive correlations between all mastitis indicators (CCS and CMT) and TIVU. TIVU showed positive correlations with CCS (r = 0.58) and CMT (r = 0.60) (P < 0.0001). The highest correlation recorded was between CCS and CMT (0.80). The relationship between TIVU and CCS was best fitted to a logarithmic equation [TIVU, °C = 31.48+ 0.38 × ln(CCS, × 103 cel/mL); R2 = 0.34; P < 0.0001). We conclude that IVT can be a valuable tool as a non-invasive method of control and detection of subclinical mastitis in dairy buffaloes, contributing to facilitate the management of diagnoses of the disease under the same conditions of milk production under study.

BANKING MEMBERS:
Presidente - 1347651 - ANGELINA BOSSI FRAGA
Interno - 1302950 - KARLOS ANTONIO LISBOA RIBEIRO JUNIOR
Externa ao Programa - 1121109 - TANIA MARTA CARVALHO DOS SANTOS
Externo à Instituição - JOSIEL BORGES FERREIRA
Externo à Instituição - JOÃO GOMES DA COSTA