Banca de QUALIFICAÇÃO: VALDEMIR DA COSTA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : VALDEMIR DA COSTA SILVA
DATE: 30/01/2023
TIME: 09:00
LOCAL: google meet
TITLE:

DEVELOPMENT AND CHARACTERIZATION OF POLYMERIC MEMBRANES ASSOCIATED WITH REDO PROPOLIS AND ALLOIN 

KEY WORDS:

Keywords: Propolis; Aloe; Polymers; Biological dressings; Antioxidant activity.

PAGES: 85
BIG AREA: Multidisciplinar
AREA: Biotecnologia
SUMMARY:

Chronic skin injuries, such as burns, ulcers, or other traumatic injuries, represent a health problem that affects millions of people around the world and causes social and economic costs. In the last twenty years, biocompatible, biodegradable, and bioabsorbable polymers have been the most used to develop membranes for wound healing. Natural polymers such as sodium carboxymethylcellulose and pectin are carriers of bioactive compounds and have a high swelling capacity. Incorporating natural products such as red propolis (PV) and aloin into membranes is a relevant strategy to enhance wound healing. PV and aloin have several pharmacological activities, such as antioxidant, anti-inflammatory, and with healing and tissue repair properties. Thus, this work aimed to develop and characterize hydrophilic polymeric membranes associated with PV and aloin. Extraction by maceration was used to obtain the hydroalcoholic extract of red propolis (EHPV) 30% (w/v). The membranes (MPV, MALO,  and MPVALO) were obtained by previously preparing a carboxymethylcellulose and pectin gel, followed by the association of EHPV and aloin. The extracts and polymeric membranes were evaluated for antioxidant activity by DPPH and FRAP methods. The determination of total phenols and flavonoids was performed by the colorimetric method of Folin-Ciocalteau and aluminum chloride respectively. Membranes associated with PV and aloin were evaluated for degradation, and compatibility by Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), and FTIR. The membranes’ morphology WAS obtained by Scanning Electron Microscopy (SEM). The degree of swelling was determined in saline solution. The polymeric membranes presented similar macroscopic morphological characteristics such as uniformity, flexibility, and resistance to handling. Phenolic compound contents of 174.2 ± 2.0 and 79.1 ± 2.5 mgEAG were found respectively in EHPV and aloin. g-1 and total flavonoids of 46.2 ± 3.2 and .9.9 ± 3.7 mgEQ. g-1. The antioxidant activity showed high activity of 91.2 ± 2.7% (EHPV), and 63.1 ± 2.5% (aloin) by the DPPH method, and by the FRAP method. Membranes loaded with extracts showed reduced antioxidant activity. Thermal analysis of the membranes by DSC, and FTIR spectra revealed membrane homogeneity with PV and aloin. MPVALO showed less homogeneity and greater thermal stability. The morphological test revealed that the membranes had a smooth surface with foci of depression from the air bubbles. The association of PV and aloin accelerates swelling and reduces the resistance of the polymeric chain. The process of obtaining the membranes did not alter the integrity of the EHPV and aloin, maintaining their physicochemical characteristics, and allowing their development and application as dressings for cutaneous wounds.

 

 

BANKING MEMBERS:
Presidente - 2120103 - MARILIA OLIVEIRA FONSECA GOULART
Interno(a) - 1247925 - ANIELLE CHRISTINE ALMEIDA SILVA
Interno(a) - 426640 - FABIANE CAXICO DE ABREU GALDINO
Externo(a) ao Programa - 1553302 - IRINALDO DINIZ BASILIO JUNIOR
Externo(a) ao Programa - 1820117 - ISIS MARTINS FIGUEIREDO
Externo(a) ao Programa - 1006306 - JADRIANE DE ALMEIDA XAVIER
Externo(a) à Instituição - TERESINHA GONÇALVES DA SILVA - UFPE