Banca de QUALIFICAÇÃO: JHONY WILLAMS GUSMÃO DO NASCIMENTO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : JHONY WILLAMS GUSMÃO DO NASCIMENTO
DATE: 19/05/2023
TIME: 09:30
LOCAL: Videoconferência
TITLE:

Liraglutide effects on the gastrointestinal tract in vitro and in vivo: relationships between motility, the immune system and cellular mechanisms involved in weight loss.

KEY WORDS:

Obesity, Liraglutide, Gastroin testinal tract, Cell viability, Cell migration.

PAGES: 50
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Obesity is a disease that presents clinical complications associated with multiple meta bolic disorders. The gastrointestinal ( tract is recognized as the largest endocrine organ in the human body, producing hormones that regulates the systemic homeostasis. Interferences in the regulation of these hormones can contribute to obesity. The maintenance of intestinal integrity and homeostasis depends on the barrier effect of the epithelium, which limits the translocation of lumi nal antigens and promotes intestinal immune regulation. Among the GI hormones, incretins stand out, especially for GLP 1. Liraglutide is a GLP 1 analogue initially available for the treatment of type 2 diabetes. Its ability to induce weight loss has made liraglutide the drug of choice for weight control, in combination with diet and exercise In addition to its known roles in the GI motility, it is suggested that GLP 1 acts as a protective factor for the integrity of the intestinal barrier, decreasing infl ammation and protecting the intestinal mucosa, although concentration dependent cytotoxic effects of these analogues have been reported. The aim of this study was to evaluate in vitro and in vivo effects of Liraglutide in the GI tract of obese rats. The in vitro stage consisted of evaluating the effects of Liraglutide on the intestinal epithelial cells of rats (IEC 6). Cells were treated with Liraglutide at concentrations 1, 0.5 and 0.25 μM for 24 hours. Cell viability, rate of apoptosis and cell necrosis were evaluated. Morphological changes and the arrangement of actin fibers in cells were also analyzed. There was no decrease in cell viability at the concentrations of 0.25, 0.5 and 1 μM. Drug treatment decreased the rate of apoptosis of IEC 6 cells compa red to control. Morphologically, some treated cells had more elongated structures and decreased cytoplasm. Treated cells showed prominent stress fibers. Regarding cell migration, there was a decrease in the percentage of closure of the cell free area, with in 24 hours, compared to non treated cells. The results showed that Liraglutide decreases the rate of apoptosis, interferes with the disposition of the actin cytoskeleton and reduces cell migration in IEC 6 cells.

BANKING MEMBERS:
Presidente - 259.657.108-01 - LUCIANA APARECIDA CORA - UNCISAL
Interno(a) - 021.848.674-03 - PEDRO DE LEMOS MENEZES - UNCISAL
Externo(a) à Instituição - MADILEINE FRANCELY AMERICO
Externo(a) à Instituição - FERNANDO GOMES ROMEIRO - UNESP